A RAPID GENOTYPING METHOD FOR AN OBLIGATE FUNGAL PATHOGEN, PUCCINIA STRIIFORMIS F.SP. TRITICI, BASED ON DNA EXTRACTION FROM INFECTED LEAF AND MULTIPLEX PCR GENOTYPING

A rapid genotyping method for an obligate fungal pathogen, Puccinia striiformis f.sp. tritici, based on DNA extraction from infected leaf and Multiplex PCR genotyping

Abstract Background Puccinia striiformis f.sp.tritici (PST), an obligate fungal pathogen causing wheat yellow/stripe rust, a serious disease, has been used to understand the evolution of crop pathogen HYALURONIC MOISTURE TREATMENT using molecular markers.However, numerous questions regarding its evolutionary history and recent migration routes stil

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Microwave-responsive gadolinium metal-organic frameworks nanosystem for MRI-guided cancer thermotherapy and synergistic immunotherapy

The clinical application of cancer immunotherapy is unsatisfied due to low response rates and systemic immune-related adverse events.Microwave hyperthermia can be used as a synergistic immunotherapy to amplify the antitumor Wardrobes effect.Herein, we designed a Gd-based metal-organic framework (Gd-MOF) nanosystem for MRI-guided thermotherapy and s

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Identification and Verification of Differential Display cDNAs Using Gene-Specific Primers and Hybridization Arrays

An accurate and streamlined approach to differential display (DD) band identification and verification is described.To minimize false positives, the strategy avoids the use Knitwear of impure Northern blot probes obtained from PCR-amplified DD bands.To increase throughput, the cloning of DD bands is replaced by a gene-specific primer approach, and

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Functional connections between cell cycle and proteostasis in the regulation of Candida albicans morphogenesis

Summary: Morphological plasticity is a key virulence trait for many fungal pathogens.For the opportunistic fungal pathogen Candida albicans, transitions among yeast, pseudohyphal, and hyphal forms are critical for virulence, because the morphotypes play distinct roles in the infection process.C.albicans morphogenesis is 7KV induced in response to m

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